| Protein name |
Chymosin B |
| Host compatibility |
Lactococcus lactis |
| Yield |
Protein did not secrete and showed no clotting activity, probably beacause of folding issues (Luerce et al., 2014)
|
| Scale (in specific example) |
Lab scale in flask (Luerce et al., 2014) *
|
| Biggest scale product has been develloped in this organism |
Lab scale in flask (Luerce et al., 2014) *
|
| Regulatory status in Europe |
Not allowed |
| Regulatory status in other parts of the world |
No FDA GRAS approval in US, not allowed in Canada |
| Companies producing this protein in this organism |
NA |
| Extra/remark |
|
| Publications/references |
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Luerce, T. D., Azevedo, M. S. P., LeBlanc, J. G., Azevedo, V., Miyoshi, A., & Pontes, D. S. (2014). RecombinantLactococcus lactisfails to secrete bovine chymosine. Bioengineered, 5(6), 363–370. https://doi.org/10.4161/bioe.36327
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Barros, R. F., De Marco, J. L., Piva, L. C., Coelho, C. M., De Moraes, L. M. P., & Torres, F. A. (2025). A comparison of different constitutive expression systems for the production of bovine chymosin in Komagataella phaffii. Journal of Dairy Science. https://doi.org/10.3168/jds.2025-26935
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Chitpinityol, S., & Crabbe, M. C. (1998). Chymosin and aspartic proteinases. Food Chemistry, 61(4), 395–418. https://doi.org/10.1016/s0308-8146(97)00090-3
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Hayes, M. G., Oliveira, J. C., McSWEENEY, P. L. H., & Kelly, A. L. (2002). Thermal inactivation of chymosin during cheese manufacture. Journal of Dairy Research, 69(2), 269–279. https://doi.org/10.1017/s0022029902005472
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Vallejo, J., Ageitos, J., Poza, M., & Villa, T. (2012). Short communication: A comparative analysis of recombinant chymosins. Journal of Dairy Science, 95(2), 609–613. https://doi.org/10.3168/jds.2011-4445
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Fährenmark, J., Lagerlund, I., Morenweiser, R., Caussette, M., Piron, E., & Ab, A. (2002, June 27). WO2002050253A1 - A method for the purification of chymosin - Google Patents. Retrieved September 4, 2025, from https://patents.google.com/patent/WO2002050253A1/enI
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